Biodiesel Synthesis through the Non-Alcohol Route using the Immobilized Lipase Enzyme from Bacillus subtilis as Biocatalyst

Biodiesel is one alternative energy that might be promising to substitute the petroleum-based fuel due its biodegradability and less toxic level. Biodiesel can synthesized through non-alcohol route by substituting alcohol with methyl acetate using lipase enzyme as Biocatalyst, which may give by-product during reaction. In this research, from Bacillus subtilis has been produced prepared in a crude, dry immobilized form. It used Biocatalyst for biodiesel synthesis. Several variables were investigated obtaining optimum condition producing biodiesel, including form of enzyme, variation molar ratio between oil acetate, concentration. The Bacillus subtilis loading was also calculated know how many percent successfully immobilized. will then compared Candida rugosa commercial lipase. Based on experimental, 1:12 concentration 2% gives highest yield among others, much 53.99%. This higher than conclusion, production.